Inhibition of Colon Cancer Cells Proliferation and Invasion by Etoposide Loaded on Nano-Suspensions Containing Black Currant Seed and Evening Primrose Oils

Formulating etoposide into a nanoemulsion based on combining two natural oils, Ribes nigrum (black currant) seed oil and organic evening primrose oils may improve its antineoplastic activity in cancer cells. The present study aimed to synthesize, characterize vitro evaluate the cytotoxic effect of formulating etoposide-loaded Colorectal Carcinoma colon anti-proliferation anti-invasion effects produced formula were investigated by cell counting kit-8 collagen-based invasion assay, respectively. Mitochondrial staining kit, annexin Fluorescein isothiocyanate double staining, nucleosome detection used discriminate stages apoptosis. According dynamic light scattering results, consisted nanodroplets with z-average diameters 144.6±5.3 nm mean surface charge -0.081±0.001 mV. Only (1.7±1.3) µM was able cause 50 % reduction cellular growth which strikingly lower than that free- (18.1±2.5 µM) around ten-fold. superior apoptotic relative cells ascertained enhanced mitochondrial depolarization, higher nucleosomal ratio increased amount early late populations. treatment impeded colorectal carcinoma (54.8±1.2) %, greater deterred only (35.1±1.2) invaded In conclusion, incorporation formulated from bioactive potentiates antitumor